trpA [2018-05-03 16:16:55]

tryptophan synthase (alpha subunit)

locus: BSU22630

pI: 4.82

mw: 29.30 kDa

protein length: 267 aa

gene length: 801 bp

function: biosynthesis of tryptophan

product: tryptophan synthase (alpha subunit)

essential: no

ec: 4.2.1.20

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.13|Biosynthesis/ acquisition of aromatic amino acids]

Gene

Coordinates: 2,371,508 → 2,372,311

The protein

Catalyzed reaction/ biological activity

• L-serine 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan glyceraldehyde 3-phosphate H₂O (according to Swiss-Prot)

Protein family

• UPF0403 family (according to Swiss-Prot)

Structure

• [PDB|1WQ5] (from ''Escherichia coli'', 35% identity, 53% similarity) [Pubmed|15667212]

Expression and Regulation

Operons

• genes: [gene|48C33A96F3B446440D4D9A86AA07AA3BA244063D|trpE]-[gene|4CD2A930753175B7F533EA6B91478577CE80A29C|trpD]-[gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]-[gene|C6872585F9BD3717A4F49E96F7684C4A5AD1760D|trpF]-[gene|AEB1F4E28C5B2AFC7FCF65721D4D1A70D6FC38EE|trpB]-[gene|155FE26C148F7022948DD7539533AB6876571862|trpA]
  description: [Pubmed|21815947]

  regulatory mechanism

  • [protein|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|MtrB]: termination/antitermination, [pubmed|1551827], in [regulon|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|MtrB regulon]

  regulation

  • not expressed if tryptophan is available ([protein|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|MtrB]) [Pubmed|1551827]
  • the mRNA is substantially stabilized upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
  • the [SW|RNA switch] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

• genes: [gene|48C33A96F3B446440D4D9A86AA07AA3BA244063D|trpE]-[gene|4CD2A930753175B7F533EA6B91478577CE80A29C|trpD]-[gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]-[gene|C6872585F9BD3717A4F49E96F7684C4A5AD1760D|trpF]-[gene|AEB1F4E28C5B2AFC7FCF65721D4D1A70D6FC38EE|trpB]-[gene|155FE26C148F7022948DD7539533AB6876571862|trpA]-[gene|4D7D62B4CDA350DCDC04E6934B5E23ECE4C57870|hisC]-[gene|DEB8358CE34F80560BE91F036659EF2C67A19623|tyrA]-[gene|CA00DFB480CBDC8AC31559958EF8E97E1E9BD4E1|aroE]
  description: [Pubmed|21815947]

  regulatory mechanism

  • [protein|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|MtrB]: termination/antitermination, [pubmed|1551827], in [regulon|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|MtrB regulon]

  regulation

  • not expressed if tryptophan is available ([protein|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|MtrB]) [Pubmed|1551827]
  • the mRNA is substantially stabilized upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]

Biological materials

Mutant

• BKE22630 (Δ[gene|155FE26C148F7022948DD7539533AB6876571862|trpA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTTCTGATGGTTGAAGAT, downstream forward: _UP4_TACAGTTTAAAATGAGGTGA
• BKK22630 (Δ[gene|155FE26C148F7022948DD7539533AB6876571862|trpA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTTCTGATGGTTGAAGAT, downstream forward: _UP4_TACAGTTTAAAATGAGGTGA

References

Reviews

• 18486479,11893063,11163353,7900177,19387555,12859215,11395405

Original publications

• 14976255,3924737,6436812,2422155,8419914,1551827,21815947